Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru
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چکیده
منابع مشابه
Biochemical and immunological characteristics of Peruvian Loxosceles laeta spider venom: neutralization of its toxic effects by anti-loxoscelic antivenoms.
This manuscript describes the general biochemical properties and immunological characteristics of Peruvian spider Loxosceles laeta venom (PLlv), which is responsible for the largest number of accidents involving venomous animals in Peru. In this work, we observed that the venom of this spider is more lethal to mice when compared with L. laeta venom from Brazil (BLlv). The LD₅₀ of PLlv was 1.213...
متن کاملA protective immune response against lethal, dermonecrotic and hemorrhagic effects of Loxosceles intermedia venom elicited by a 27-residue peptide.
Antibodies raised against recombinant Loxosceles intermedia dermonecrotic protein isoform 1 (rLiD1) display neutralizing capacity for the L. intermedia whole venom. We previously found that an immunodominant continuous B-cell epitope, recognized by these antibodies corresponds to a region of the protein known to be involved in the active site. In this study, we extend previous work by preparing...
متن کاملLoxosceles laeta venom in rabbits
5 Guilherme Martins1, Maira Oliveira1, Ana Flávia Botelho1, Conrado Gamba2, Clara Duarte3, 6 Adriane Bicalho1, Geovanni Cassali2, Alfredo Goes2, Carlos Chávez-Olórtegui2, Benito 7 Soto-Blanco1 and Marilia Melo1* 8 9 1 Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas 10 Gerais, Brazil; [email protected] (GM); [email protected] (MO);...
متن کامل[Effectiveness of a repellent paint against the spider Loxosceles laeta].
BACKGROUND Loxoscelism is a severe reaction to the bite of the spider Loxosceles laeta. In recent years, a paint with repellent properties has been promoted in the commerce. However, there are no reports of experiments evaluating its effectiveness. AIM To evaluate experimentally the repellent properties of a paint against Loxosceles laeta. MATERIAL AND METHODS Males, females and nymphs of L...
متن کاملA Novel Hyaluronidase from Brown Spider (Loxosceles intermedia) Venom (Dietrich's Hyaluronidase): From Cloning to Functional Characterization
Loxoscelism is the designation given to clinical symptoms evoked by Loxosceles spider's bites. Clinical manifestations include skin necrosis with gravitational spreading and systemic disturbs. The venom contains several enzymatic toxins. Herein, we describe the cloning, expression, refolding and biological evaluation of a novel brown spider protein characterized as a hyaluronidase. Employing a ...
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ژورنال
عنوان ژورنال: Toxicon
سال: 2015
ISSN: 0041-0101
DOI: 10.1016/j.toxicon.2014.10.023